Composite

Part:BBa_K4032105

Designed by: Yumika Sato   Group: iGEM21_Gunma   (2021-09-30)
Revision as of 09:29, 19 October 2021 by Tsugumi (Talk | contribs)


lacI+RBS+DegP+double terminator

This part contains sequences of DegP protein.

This part is the addition of a lac promoter and RBS and double terminator to BBa_K40320xx. Lac promoter and RBS and double terminator were used as contained in BBa_J04450.

DegP is a type of heat shock protein in E.coli. Heat shock protein is a family of proteins whose expression is induced by SOS responses such as heat. It is also a type of serine protease due to the presence of serine residues in the active center. In particular, it recognizes and degrades peptides whose structure has been damaged by heat or misfolding.

This part was created by the In-Fusion method.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1364
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1364
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1364
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1364
    Illegal AgeI site found at 2206
    Illegal AgeI site found at 2318
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None