Part:BBa_K4032105

lacI+RBS+DegP+double terminator

Contents:

・The lac promoter from BBa_R0010

・The RBS from BBa_B0034

・The gene of DegP protein from BBa_K4032002

・The double terminator from BBa_B0015

Usage and Biology

DegP is a type of heat shock protein in E. coli. Heat shock protein is a family of proteins whose expression is induced by SOS responses such as heat.

It is also a type of serine protease in terms of the presence of serine residues in the active center.

In particular, it recognizes and degrades peptides whose structure has been damaged by heat or misfolding.

Design

The lac promoter, the RBS and the double terminator are added to BBa_K4032002, forming this part.

The lac promoter, the RBS and the double terminator are from BBa_J04450.

This part was created by the In-Fusion method.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 1364
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 1364
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 1364
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 1364
  Illegal AgeI site found at 2206
  Illegal AgeI site found at 2318
  
• 1000
  COMPATIBLE WITH RFC[1000]