Translational_Unit
RBS amyRFP

Part:BBa_K4032006

Designed by: Tsugumi Ohzeki   Group: iGEM21_Gunma   (2021-09-30)
Revision as of 09:16, 19 October 2021 by Tsugumi (Talk | contribs)


RBS+amylase-RFP

This part contains sequences of fuse protein of amylase and RFP.

This part is the addition of RBS to BBa_K40320xx. RBS were used as contained in BBa_K523006.

This enzyme hydrolyzes of (1-4)-alpha-D-glycosidic linkages in polysaccharides containing three or more (1-4)-alpha-linked D-glucose units. Red fluorescence of RFP is observed under the fluorescence microscope.

We also created BBa_K40321xx by adding lac promoter and double terminator to this part.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1233
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 369
    Illegal AgeI site found at 1534
    Illegal AgeI site found at 2613
    Illegal AgeI site found at 2725
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None