Coding
Part:BBa_K3952001:Design
Designed by: B. Liam, D. Amanda, R. Jazmine, S. Brianna Group: iGEM21_SUNY_Oneonta (2021-10-01)
APOB-100 (Cholesterol Deficiency Allele)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 330
Illegal XhoI site found at 591 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 853
Design Notes
This is the positive control of the APOB gene that will be used within a Flappase genetic testing system.
A SpeI restriction site was found at position 853. To adhere to RCF1000 assembly, a silent mutation was applied to the codon located within positions 855 and 857. The original codon was AGA and was altered to AGG.
Source
The source of the APOB-100 sequence is the genomic sequence from Bos taurus, apolipoprotein B genome.
References
Through the assistance provide by Team Rochester, we were able to acquire the ERV2-1 sequence from their subscription to Repbase. Repbase is a database consisting of repetitive DNA elements.