Part:BBa_K3718003

sgRNA for arcA with terminator - transcription template

Single guide RNA targeting arcA, for knock-out

crRNA

Through the results returned by CHOPCHOP, our team selected the crRNA at rank no.2, which is positioned at about one-fifth into the arcA gene. The arcA protein is expected to be dysfunctioned. The CG content is 40% and its efficiency is predicted at 70.41%.

tracrRNA and T7 terminator

The design of tracrRNA and T7 terminator is referenced from the 2017 IGEM team iGEM17_BGIC-Union, part BBa_K2371006.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal prefix found in sequence at 1
  Illegal suffix found in sequence at 171
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1
  Illegal SpeI site found at 172
  Illegal PstI site found at 186
  Illegal NotI site found at 7
  Illegal NotI site found at 179
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found in sequence at 1
  Illegal suffix found in sequence at 172
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found in sequence at 1
  Illegal XbaI site found at 16
  Illegal SpeI site found at 172
  Illegal PstI site found at 186
  
• 1000
  COMPATIBLE WITH RFC[1000]