RNA

Part:BBa_K3718003

Designed by: Chan Pui Ching   Group: iGEM21_Hong_Kong_UCCKE   (2021-09-30)


sgRNA for arcA with terminator - transcription template

Single guide RNA targeting arcA, for knock-out

crRNA

Through the results returned by CHOPCHOP, our team selected the crRNA at rank no.2, which is positioned at about one-fifth into the arcA gene. The arcA protein is expected to be dysfunctioned. The CG content is 40% and its efficiency is predicted at 70.41%.

tracrRNA and T7 terminator

The design of tracrRNA and T7 terminator is referenced from the 2017 IGEM team iGEM17_BGIC-Union, part BBa_K2371006.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 171
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 172
    Illegal PstI site found at 186
    Illegal NotI site found at 7
    Illegal NotI site found at 179
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 172
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 172
    Illegal PstI site found at 186
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None