Part:BBa_K3893028

AHL induced expression of GFP (including luxR constitutive expression)

AHL induced expression of GFP (including luxR constitutive expression)

File:T--Ecuador--K3893087 sbolv.png

Figure 1. SBOL Visual representation of the device

This devise was used to characterize the pLux promoter AHL induced transcriptional unit assembled with a one-pot Level 1 Golden Gate reaction using BsaI type IIS endonuclease.

This composite part, together with K3893027, is a component of the intermediate part K3893029 to assemble the release system device (K3893030) of our Agrobactory 593. It is used to constitutively express LuxI protein, the AHL synthase, a fundamental piece of our population oscillator. release device.

This transcriptional unit is composed of the following standardized composite parts from Valencia_UPV 2018 iGEM Team [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]:

• BBa_K2656122: a Level 1 transcriptional unit expresing GFP under the control of the pux promoter (Golden Braid compatible)
• BBa_K2656114: a Level 1 transcriptional unit constitutively expressing luxR gene (Golden Braid compatible)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 956
  Illegal NheI site found at 979
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 956
  Illegal NheI site found at 979
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]