Primer
pTF_fbp-fw
Part:BBa_K4047005
Designed by: An Nguyen, Avery Imes Group: iGEM21_MiamiU_OH (2021-09-07)
Revision as of 18:27, 4 October 2021 by Anniequyan (Talk | contribs)
primerTF_fbp-foward
Forward primer for FBPase
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Long Description
This is the forward primer to amplify the gene for fructose-1,6-bisphosphatase. The overlap sequence (red) allows proper Gibson assembly with the pGEM-tal+fbp backbone. The annealing portion (all caps) allows annealing to the WT S. elongatus PCC 7942 genome for proper amplification.
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Categories
Parameters
//primer/part
None |