Part:BBa_K3733005

J23106a:Improved promoter of J23106

J23106a is a constitutive promoter obtained by mutating <a url="https://parts.igem.org/Part:BBa_J23106"> J23106</a>. The strength of this promoter is much weaker than J23106.

Functional Parameters

To characterize this part, J23106a and J23106 were cloned into pSC101 vector separately. We chose <a url="https://parts.igem.org/Part:BBa_K3733012">neGFP </a> as the reporter. Plasmids were transferred into E.coli DH5α. The E. coli strain was cultured overnight, and the relative intensity of J23106a was evaluated by measuring RFU/OD₆₀₀ under the control of two different promoters. The relative fluorescence unit (RFU) was determined by Synergy H1 microplate reader. 

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Figure 2. Comparison of relative strength between J23106 and J23106a.The microplate reader gain adopts 50

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]