Composite

Part:BBa_K3755012:Design

Designed by: Qi Xin   Group: iGEM21_ShanghaiTech_China   (2021-09-30)
Revision as of 08:33, 9 October 2021 by HeteroauXin (Talk | contribs) (→‎Source)


AKTK tag-his tag-2*Mfp5 CDS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 46
    Illegal XhoI site found at 514
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is a complete CDS for 2 repeats of Mfp5 protein expression. However, the start codon and stop codon is not contained in the sequence. So please add the start codon and the stop codon to the beginning and the end of the sequence before you put this part into the protein expression plasmid(which we recommend pET28).

Source

From literature of Eugene Kim's team from Washington University in St. Louis.

References