Composite

Part:BBa_K3755012:Design

Designed by: Qi Xin   Group: iGEM21_ShanghaiTech_China   (2021-09-30)


AKTK tag-his tag-2*Mfp5 CDS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 46
    Illegal XhoI site found at 514
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is a complete CDS for 2 repeats of Mfp5 protein expression. However, the start codon and stop codon is not contained in the sequence. So please add the start codon and the stop codon to the beginning and the end of the sequence before you put this part into the protein expression plasmid(which we recommend pET28).

Source

From literature of Eugene Kim's team from Washington University in St. Louis.

References

[1]Danner EW, Kan Y, Hammer MU, Israelachvili JN, Waite JH. Adhesion of mussel foot protein Mefp-5 to mica: an underwater superglue. Biochemistry. 2012 Aug 21;51(33):6511-8. doi: 10.1021/bi3002538. Epub 2012 Aug 8. PMID: 22873939; PMCID: PMC3428132.
[2]Yang B, Song J, Jiang Y, Li M, Wei J, Qin J, Peng W, Lasaosa FL, He Y, Mao H, Yang J, Gu Z. Injectable Adhesive Self-Healing Multicross-Linked Double-Network Hydrogel Facilitates Full-Thickness Skin Wound Healing. ACS Appl Mater Interfaces. 2020 Dec 30;12(52):57782-57797. doi: 10.1021/acsami.0c18948. Epub 2020 Dec 18. PMID: 33336572.
[3]Huang J, Li H, Wang Q. [Development of double-component rapid curing bioadhesive]. Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2018 Dec 25;35(6):921-927. Chinese. doi: 10.7507/1001-5515.201805045. PMID: 30583318.
[4]Liang Y, Zhao X, Hu T, Han Y, Guo B. Mussel-inspired, antibacterial, conductive, antioxidant, injectable composite hydrogel wound dressing to promote the regeneration of infected skin. J Colloid Interface Sci. 2019 Nov 15;556:514-528. doi: 10.1016/j.jcis.2019.08.083. Epub 2019 Aug 24. PMID: 31473541.
[5]Yang Y, Liang Y, Chen J, Duan X, Guo B. Mussel-inspired adhesive antioxidant antibacterial hemostatic composite hydrogel wound dressing via photo-polymerization for infected skin wound healing. Bioact Mater. 2021 Jun 23;8:341-354. doi: 10.1016/j.bioactmat.2021.06.014. PMID: 34541405; PMCID: PMC8427086.
[6]Kord Forooshani P, Lee BP. Recent approaches in designing bioadhesive materials inspired by mussel adhesive protein. J Polym Sci A Polym Chem. 2017 Jan 1;55(1):9-33. doi: 10.1002/pola.28368. Epub 2016 Oct 11. PMID: 27917020; PMCID: PMC5132118.
[7]Lee BP, Messersmith PB, Israelachvili JN, Waite JH. Mussel-Inspired Adhesives and Coatings. Annu Rev Mater Res. 2011 Aug 1;41:99-132. doi: 10.1146/annurev-matsci-062910-100429. PMID: 22058660; PMCID: PMC3207216.
[8]Hwang DS, Yoo HJ, Jun JH, Moon WK, Cha HJ. Expression of functional recombinant mussel adhesive protein Mgfp-5 in Escherichia coli. Appl Environ Microbiol. 2004 Jun;70(6):3352-9. doi: 10.1128/AEM.70.6.3352-3359.2004. PMID: 15184131; PMCID: PMC427802.
[9]Kim E, Dai B, Qiao JB, Li W, Fortner JD, Zhang F. Microbially Synthesized Repeats of Mussel Foot Protein Display Enhanced Underwater Adhesion. ACS Appl Mater Interfaces. 2018 Dec 12;10(49):43003-43012. doi: 10.1021/acsami.8b14890. Epub 2018 Nov 27. PMID: 30480422.