Composite
Part:BBa_K3425060:Design
Designed by: Tereza Hubackova Group: iGEM20_UofUppsala (2020-10-25)
T7 polymerase regulated Juniper GFP substrate for Qβ replicase
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 550
Illegal XbaI site found at 83 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 550
Illegal NheI site found at 859 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 550
Illegal BglII site found at 86
Illegal BamHI site found at 759
Illegal XhoI site found at 90
Illegal XhoI site found at 890 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 550
Illegal XbaI site found at 83 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 550
Illegal XbaI site found at 83 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
TBD
Source
TBD