Composite

Part:BBa_K3657034

Designed by: Jannik Berger   Group: iGEM20_Heidelberg   (2020-10-21)
Revision as of 02:06, 28 October 2020 by Liza ch (Talk | contribs)


RNA AUAGAU binding Pumby protein coupled with C-Terminal GFP

This is a fusion protein containing AUAGAU binding Pumby Protein (BBa_K3657004) and C-terminal Split-GFP (BBa_K3657028).

Usage and Biology

AUAGAU binding Pumby Protein binds specifically the AUAGAU RNA sequence(BBa_K3657015). Pumby Proteins can be designed to bind a specific RNA sequence.

C-terminal Split-GFP is a protein that combines to a fluorescent complex when in proximity of N-terminal Split-GFP (BBa_K3657027).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 184
    Illegal XhoI site found at 224
    Illegal XhoI site found at 449
    Illegal XhoI site found at 634
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None