Part:BBa_K3505005

TetR- Tet repressor GB compatible with B3-B5

TetR- tetR Coding region for Tet repressor without an RBS .

Usage and Biology

Figure 1.The overhangs of this part in the GoldenBraid Grammar.

Figure 2. The level 0 module : pupd2- TetR (illustration from SnapGene)

Verification of Cloning

Fig.3:(U=Uncut C=Cut) Restriction digestion of TetR (C2 -C4) with : SpeI + EcoRI (C2 - C4) , Expected bands : 2047 + 680 bp Positive result : C4

Source

Synthesized by IDT.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 4
  
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 4
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 4
  
• 1000
  COMPATIBLE WITH RFC[1000]