Part:BBa_K3629020

Yarrowia lipolytica TRP2 Gene

Anthranilate synthase component 1 (TRP2) coding sequence Yarrowia lipolytica .

Usage and Biology

Anthranilate synthase component 1 is the first enzyme involved in the biosynthesis of tryptophan This is a very important enzyme in the pathway as it serves as a regulatory point in the biosynthesis pathway (1). The protein has a tryptophan binding domain which allows for downregulation of the pathway through negative-feedback inhibition of the enzyme. Expression of this enzyme especially on a strong promoter could be used to up-regulate tryptophan synthesis and produce a tryptophan overproducing strain.

See BBa_K3629024 for a high expression construct using this coding sequence.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 943
  Illegal NgoMIV site found at 1213
  Illegal NgoMIV site found at 1294
  Illegal NgoMIV site found at 1393
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 283
  Illegal BsaI.rc site found at 544
  Illegal BsaI.rc site found at 1441
  

References

1. [online] https://www.genome.jp/kegg-bin/show_pathway?yli00400 (Accessed October 26, 2020)