Part:BBa_K3629020

Yarrowia lipolytica TRP2 Gene

Anthranilate synthase component 1 (TRP2) coding sequence Yarrowia lipolytica .

Usage and Biology

Anthranilate synthase component 1 is the first enzyme involved in the biosynthesis of tryptophan This is a very important enzyme in the pathway as it serves as a regulatory point in the biosynthesis pathway (1). The protein has a tryptophan binding domain which allows for downregulation of the pathway through negative-feedback inhibition of the enzyme. Expression of this enzyme especially on a strong promoter could be used to up-regulate tryptophan synthesis and produce a tryptophan overproducing strain.

Design

Below is the codon-optimized sequence of Yarrowia lipolytica TRP2 for use in any expression construct. However, we have also made our own expression construct BBa_K3629024, designed to allow for optimal expression of TRP2 in Yarrowia lipolytica using the TEfin promoter BBa_K3629001 and the XPR2 terminator BBa_K3629004.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 943
  Illegal NgoMIV site found at 1213
  Illegal NgoMIV site found at 1294
  Illegal NgoMIV site found at 1393
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 283
  Illegal BsaI.rc site found at 544
  Illegal BsaI.rc site found at 1441
  

References

1.KEGG PATHWAY: Phenylalanine, tyrosine and tryptophan biosynthesis - Yarrowia lipolytica. (n.d.). Retrieved October 26, 2020, from https://www.genome.jp/kegg-bin/show_pathway?yli00400