Regulatory
Part:BBa_K3599018
Designed by: Zijia Liu Group: iGEM20_TPR_China (2020-10-24)
Pa
Overview
An induced promoter which could be regulated by transcription factor PaaX (BBa_K1031710).
Sequence And Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design & Experience
In iGEM2020 TPR_China Project, an aromatic sensor was constructed using Pa promoter, the eGFP (BBa_K3599016) and the sfGFP (BBa_K3599017) were both used as downstream reporters to form the sensor devices. To sense the aromatic compounds, PaaX under J23114 constitutive promoter was used together.
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Categories
Parameters
None |