Reporter

Part:BBa_K3380153:Design

Designed by: Alexandru Popov   Group: iGEM20_Edinburgh   (2020-10-08)
Revision as of 16:31, 23 October 2020 by Alexandru popov (Talk | contribs)


Broccoli fluorescent RNA aptamer


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

Source

Filonov et al. 2014 used directed evolution to obtain the 49 nucleotides Broccoli fluorescent RNA aptamer.

References

Filonov, G.S., Moon, J.D., Svensen, N. and Jaffrey, S.R., 2014. Broccoli: rapid selection of an RNA mimic of green fluorescent protein by fluorescence-based selection and directed evolution. Journal of the American Chemical Society, 136(46), pp.16299-16308.