Composite

Part:BBa_K3425051:Design

Designed by: Tereza Hubackova   Group: iGEM20_UofUppsala   (2020-10-17)
Revision as of 15:29, 17 October 2020 by Tereza H (Talk | contribs) (→‎Design Notes)


β-subunit of Qβ replicase with cleavable degradation tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1314
    Illegal XhoI site found at 822
    Illegal XhoI site found at 988
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Since the part is designed to be cloned into the Level 0 backbone pSB1C00 using Type IIS iGEM assembly standard, it lacks ATG start codon. You can read more about how to use this cloning standard in <a href="">Type IIS guide</a> of Uppsala 2020 team.

This part was synthesized with internal stop codon as a control construct. Functional construct with destabilized

Source

Synthesized.

References