Composite

Part:BBa_K3425051:Design

Designed by: Tereza Hubackova   Group: iGEM20_UofUppsala   (2020-10-17)


β-subunit of Qβ replicase with cleavable degradation tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1314
    Illegal XhoI site found at 822
    Illegal XhoI site found at 988
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

BBa_K3425051 was designed to be cloned into the Level 0 backbone pSB1C00 using Type IIS iGEM assembly standard, and therefore it lacks the start codon. You can read more about how to use this cloning standard in Type IIS guide of Uppsala 2020 team.

This part was synthesized with internal stop codon between the β-subunit and the cleavage signal. It served as a control construct.

Source

Synthesized without scars.

References



QB replicase
[1]
Yao, Y., Zhang, W., Zhang, M., Jin, S., Guo, Y., Zu, Y., Ren, K., Wang, K., Chen, G., Lou, C., and Wu, Q. (2019) A Direct RNA-to-RNA Replication System for Enhanced Gene Expression in Bacteria. ACS Synth. Biol. 8, 1067–1078
Note, that the authors declared the following competing financial interest(s): Y. Y., W. Z., and Q. W. have filed a patent on RNA replication system and its usage.