Composite

Part:BBa_K3408009:Design

Designed by: Yunong Li   Group: iGEM20_NAU-CHINA   (2020-10-08)
Revision as of 12:54, 8 October 2020 by Registry (Talk | contribs)

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PliaG-trigger RNA-B0015-PCⅠ-switch RNA-GFP-B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 239
    Illegal BglII site found at 461
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1190


Design Notes

We used this part to verify the feasibility of our IPTG induction system in our engineered Bacillus subtilis.


Source

We designed it by combining some basic parts.

References