Part:BBa_K3408009:Design

P_liaG-trigger RNA-B0015-P_CⅠ-switch RNA-GFP-B0015

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 239
  Illegal BglII site found at 461
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1190
  

Design Notes

We wanted to make sure that our toehold switch could work normally by checking if there was GFP expressed in the engineered bacteria.

Source

We designed it with our basic part trigger RNA (BBa_K3408002) and switch RNA (BBa_K3408003).