Coding

Part:BBa_K3610002:Design

Designed by: Jonas Sebastian Trottmann   Group: iGEM20_UZurich   (2020-09-01)
Revision as of 18:18, 30 September 2020 by Jtrott (Talk | contribs) (→‎Source)


BAK1 from Arabidopsis thaliana without signal sequence


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1485
    Illegal PstI site found at 575
    Illegal PstI site found at 620
    Illegal PstI site found at 910
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1485
    Illegal PstI site found at 575
    Illegal PstI site found at 620
    Illegal PstI site found at 910
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1485
    Illegal PstI site found at 575
    Illegal PstI site found at 620
    Illegal PstI site found at 910
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1485
    Illegal PstI site found at 575
    Illegal PstI site found at 620
    Illegal PstI site found at 910
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The signal sequence was not cleaved off in-frame. Further it does not contain a start codon, it therefore can not be expressed without linking it to other parts which do.


Source

Cyril Zipfel Lab at UZH, Switzerland

References