Part:BBa_K3610002:Design
BAK1 from Arabidopsis thaliana without signal sequence
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 1485
Illegal PstI site found at 575
Illegal PstI site found at 620
Illegal PstI site found at 910 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 1485
Illegal PstI site found at 575
Illegal PstI site found at 620
Illegal PstI site found at 910 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 1485
Illegal PstI site found at 575
Illegal PstI site found at 620
Illegal PstI site found at 910 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 1485
Illegal PstI site found at 575
Illegal PstI site found at 620
Illegal PstI site found at 910 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The signal sequence of the full length receptor (Part:BBa_K3610001) was not cleaved off in-frame. Further it does not contain a start codon, it therefore can not be expressed without linking it to other parts which do.
Source
Cyril Zipfel Lab at UZH, Switzerland
References
Chinchilla, Delphine; Zipfel, Cyril; Robatzek, Silke; Kemmerling, Birgit; Nürnberger, Thorsten; Jones, Jonathan D. G. et al. (2007): A flagellin-induced complex of the receptor FLS2 and BAK1 initiates plant defence. In: Nature 448 (7152), S. 497–500. DOI: 10.1038/nature05999.
Macho, Alberto P.; Zipfel, Cyril (2014): Plant PRRs and the Activation of Innate Immune Signaling. In: Molecular Cell 54 (2), S. 263–272. DOI: 10.1016/j.molcel.2014.03.028.
Yan, Liming; Ma, Yuanyuan; Liu, Dan; Wei, Xiaochao; Sun, Yuna; Chen, Xiaoyue et al. (2012): Structural basis for the impact of phosphorylation on the activation of plant receptor-like kinase BAK1. In: Cell Res 22 (8), S. 1304–1308. DOI: 10.1038/cr.2012.74.
Chinchilla, Delphine; Shan, Libo; He, Ping; Vries, Sacco de; Kemmerling, Birgit (2009): One for all: the receptor-associated kinase BAK1. In: Trends Plant Sci 14 (10), S. 535–541. DOI: 10.1016/j.tplants.2009.08.002.