Generator
Part:BBa_K143082
Designed by: Chris Hirst Group: iGEM08_Imperial_College (2008-10-27)
Pveg-spoVG RFP expression construct
RFP gene under expresson of the Pveg promoter and spoVG RBS of B. subtilis, with a chloramphenicol resistance marker for ease of selection.
Also contains the 5' and 3' amyE integration sequences to allow integration into B. subtilis at the amyE locus.
This device was used by the Imperial iGEM 2008 team to characterise the Pveg promoter and spoVG RBS (Combined part BBa_K143053) as part of the Biofabricator subtilis project.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2136
Illegal AgeI site found at 2248 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//chassis/prokaryote/bsubtilis
None |