Composite

Part:BBa_K3059509:Design

Designed by: Beteel Abu-Ageel   Group: iGEM19_William_and_Mary   (2019-10-21)
Revision as of 17:41, 21 October 2019 by Registry (Talk | contribs)

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dsRed-exp α_H - sequence confirmed


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 388
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 388
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 388
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 388
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 41
    Illegal BsaI.rc site found at 735


Design Notes

Source

Synthesized via IDT; used designer primers to add Pad sequences, BsaI cutsites, and sticky ends.

References