Composite

Part:BBa_K3059509:Design

Designed by: Beteel Abu-Ageel   Group: iGEM19_William_and_Mary   (2019-10-21)


dsRed-exp α_H - sequence confirmed


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 388
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 388
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 388
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 388
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 41
    Illegal BsaI.rc site found at 735


Design Notes

In the system designed by Karig et al, dsRed-express serves as a reporter to indicate that the first signalling pathway has been activated, leading to production of the activator and inhibitor signals.

Source

Synthesized via IDT; used designer primers to add Pad sequences, BsaI cutsites, and sticky ends.

References

1. Karig, D., Martini, K. M., Lu, T., Delateur, N. A., Goldenfeld, N., & Weiss, R. (2018). Stochastic Turing patterns in a synthetic bacterial population. Proceedings of the National Academy of Sciences, 115(26), 6572-6577. doi:10.1073/pnas.1720770115

2. Karig, D. K. (2007). Engineering multi-signal synthetic biological systems. (Doctoral dissertation). Princeton University, USA.