RNA

Part:BBa_K3015004

Designed by: Valentina Stuchlik   Group: iGEM19_BOKU-Vienna   (2019-10-13)
Revision as of 23:24, 21 October 2019 by Lenti (Talk | contribs)


Theophylline_Riboswitch (transcriptional)

The iGEM Team BOKU-Vienna improved the Part BBa_K784005 by designing a Theophylline Riboswitch that works in a transcriptional way, instead of a translational way. The coding sequence does not have to be scarless fused to the Riboswitch, which makes cloning easier.
Consists of an Aptamer-sequence, a spacer region, a terminator and a U-Stretch.
To test the probabilities of the Riboswitch we conducted measurements by fusing the Ribswitch upstream to a Promotor and downstream to an RBS and GFP. You can find those measurements on the Registry-Page of the described Composite Part BBa_K3015007

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None