Part:BBa_K3071009:Design
Phage shock protein F transcriptional activation domain fused with Clp (pspF TAD-Clp)
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 907
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 910
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 907
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 907
Illegal NgoMIV site found at 985
Illegal AgeI site found at 90
Illegal AgeI site found at 118
Illegal AgeI site found at 256 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
-
Source
Escherichia coli K12 NC_000913 & Xanthomonas campestris pv. camperstris
References
Chen, C. H., Lin, N. T., Hsiao, Y. M., Yang, C. Y., & Tseng, Y. H. (2010). Two non-consensus Clp binding sites are involved in upregulation of the gum operon involved in xanthan polysaccharide synthesis in Xanthomonas campestris pv. campestris. Research in microbiology, 161(7), 583-589.
Wigneshweraraj, S., Bose, D., Burrows, P. C., Joly, N., Schumacher, J., Rappas, M., ... & Buck, M. (2008). Modus operandi of the bacterial RNA polymerase containing the σ54 promoter‐specificity factor. Molecular microbiology, 68(3), 538-546.
Zhou, Y., Asahara, H., Schneider, N., Dranchak, P., Inglese, J., & Chong, S. (2014). Engineering bacterial transcription regulation to create a synthetic in vitro two-hybrid system for protein interaction assays. Journal of the American Chemical Society, 136(40), 14031-14038.