Coding

Part:BBa_K3071009:Design

Designed by: Leung Hei Man   Group: iGEM19_Hong_Kong-CUHK   (2019-10-08)


Phage shock protein F transcriptional activation domain fused with Clp (pspF TAD-Clp)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 907
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 910
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 907
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 907
    Illegal NgoMIV site found at 985
    Illegal AgeI site found at 90
    Illegal AgeI site found at 118
    Illegal AgeI site found at 256
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

-


Source

Escherichia coli K12 NC_000913 & Xanthomonas campestris pv. camperstris

References

Chen, C. H., Lin, N. T., Hsiao, Y. M., Yang, C. Y., & Tseng, Y. H. (2010). Two non-consensus Clp binding sites are involved in upregulation of the gum operon involved in xanthan polysaccharide synthesis in Xanthomonas campestris pv. campestris. Research in microbiology, 161(7), 583-589.

Dworkin, J., Jovanovic, G., & Model, P. (1997). Role of upstream activation sequences and integration host factor in transcriptional activation by the constitutively active prokaryotic enhancer-binding protein PspF. Journal of molecular biology, 273(2), 377-388.

Rappas, M., Schumacher, J., Beuron, F., Niwa, H., Bordes, P., Wigneshweraraj, S., ... & Zhang, X. (2005). Structural insights into the activity of enhancer-binding proteins. Science, 307(5717), 1972-1975.

Wigneshweraraj, S., Bose, D., Burrows, P. C., Joly, N., Schumacher, J., Rappas, M., ... & Buck, M. (2008). Modus operandi of the bacterial RNA polymerase containing the σ54 promoter‐specificity factor. Molecular microbiology, 68(3), 538-546.

Zhou, Y., Asahara, H., Schneider, N., Dranchak, P., Inglese, J., & Chong, S. (2014). Engineering bacterial transcription regulation to create a synthetic in vitro two-hybrid system for protein interaction assays. Journal of the American Chemical Society, 136(40), 14031-14038.