Coding

Part:BBa_K3142013

Designed by: Luo Xiaolin   Group: iGEM19_SZPT-CHINA   (2019-10-13)
Revision as of 19:24, 19 October 2019 by Xiaolin (Talk | contribs)


acmA

what it is：

what it does：

how to use it in their projects：


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 627
    Illegal PstI site found at 151
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 151
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 579
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 627
    Illegal PstI site found at 151
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 627
    Illegal PstI site found at 151
  • 1000
    COMPATIBLE WITH RFC[1000]


SZPT-China

To ensure that the transgenic bacteria are not harmful to the environment, we constructed the autolytic enzyme gene acmA of the lactic acid bacteria downstream of the glucose starvation promoter. The results of the construction of the recombinant plasmid pMG36e-pα-crp-acmA named pMG36e-P-a are shown in Fig. 18. The results of LAB MG1363 and LAB MG1363 with pMG36e-p - a after induction of recombinant bacteria by different glucose concentrations are shown in Fig. 18. The results showed that when the concentration of glucose in the environment was less than 0.05%, the growth of recombinant bacteria was significantly inhibited.

P-a.png
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