Coding

Part:BBa_K3142013

Designed by: Luo Xiaolin   Group: iGEM19_SZPT-CHINA   (2019-10-13)


acmA

AcmA is an Endo-βN-acetylmuramidase that dissociates the reducing group of N-acetylmuramic acid from Gram-positive bacteria to autolyze the lactic acid bacteria.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 627
    Illegal PstI site found at 151
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 151
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 579
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 627
    Illegal PstI site found at 151
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 627
    Illegal PstI site found at 151
  • 1000
    COMPATIBLE WITH RFC[1000]


SZPT-China

The results of LAB MG1363 and LAB MG1363 with pMG36e-p - a after induction of recombinant bacteria by different glucose concentrations are shown in Fig. 1. The results showed that when the concentration of glucose in the environment was less than 0.05%, the growth of recombinant bacteria was significantly inhibited.

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Reference

  • Coolbear, T., Pillidge, C. J., & Crow, V. L. (1994). The diversity of potential cheese ripeningcharacteristics of lactic acid starter bacteria: Resistance to cell lysis and levels of cellular distribution of proteinase activities. International Dairy Journal, 4,697–721.
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