Composite

Part:BBa_K3196022

Designed by: Hao Qiu   Group: iGEM19_HUST-China   (2019-10-16)
Revision as of 07:29, 20 October 2019 by Zyz2018hust (Talk | contribs)


AOX1-Kozak-FLO10 pro-VP-His tag-AOX1 Terminator

FLO10 αpro is a combined signal peptide, which enhance the enzyme activity 3 times. VP can catalyze lignin.

Characterization

This is a four section for degrade and transfer lignin part.FLO10 pro is the combination of FLO10 and leading peptide from α-MF, an efficient signal peptide with special leading peptide.[1] It can help the peptide chain of VP be transferred out cells.
Figure1. T--HUST--China--2019-FLO10-αproVP

DNA Gel Electrophoretic

After we link FLO10 αpro and VP successfully, we run the PCR with an intention to confirm the expression of VP. As the figure shows, we get the genetic stripe about 1101 bp which means the PCR is successful.

Figure1. This figure shows the most possible kozak consensus sequence.

Reference

[1]Effect of signal peptides on the expression of laccase in Pichia pastoris Shi Yiping , Zhou Xue , Hu Meirong , Lin Jianhui , Tao Yong , Huang Jianzhong Acta Microbiologica Sinica 54(12) :1446 － 1452; 4 December 2014 ISSN 0001 － 6209; CN 11 － 1995 /Q

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 937
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None