Composite

Part:BBa_K3196022

Designed by: Hao Qiu   Group: iGEM19_HUST-China   (2019-10-16)


AOX1-Kozak-FLO10 pro-VP-His tag-AOX1 Terminator

FLO10 αpro is a combined signal peptide, which enhance the enzyme activity 3 times. VP can catalyze lignin.

Characterization

This is a four section for degrade and transfer lignin part.FLO10 pro is the combination of FLO10 and leading peptide from α-MF, an efficient signal peptide with special leading peptide.[1] It can help the peptide chain of VP be transferred out cells.
Figure1. T--HUST--China--2019-FLO10-αproVP

Reference

[1]Effect of signal peptides on the expression of laccase in Pichia pastoris Shi Yiping , Zhou Xue , Hu Meirong , Lin Jianhui , Tao Yong , Huang Jianzhong Acta Microbiologica Sinica 54(12) :1446 － 1452; 4 December 2014 ISSN 0001 － 6209; CN 11 － 1995 /Q

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 937
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None