Regulatory
miR663b-ta

Part:BBa_K3179000:Design

Designed by: Jiaxian Xu   Group: iGEM19_SYSU-CHINA   (2019-10-13)
Revision as of 17:57, 19 October 2019 by Bigxushen (Talk | contribs) (→‎References)


miR663b-target


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We constructed miR663b-target with 2kt-E1A and miR885-5p-target so that E1A cannot be translated if a high level of miRNA663b existing in the cell.

Source

MiRNA 663b-target is constructed bearing four repeats of miRNA 663b, and each repeat added with ATG. Extra bases which can be devisable by three were added in order to separate ATGs.

References

1 Bartel D P. MicroRNAs: target recognition and regulatory functions[J]. cell, 2009, 136(2): 215-233.