Composite
INT7TLU1

Part:BBa_K3254027

Designed by: Yeqing Zong, LAW,Ki Yuk Sacheel   Group: iGEM19_GENAS_China   (2019-10-18)
Revision as of 05:38, 18 October 2019 by Zongyeqing (Talk | contribs)


Translational Unit for Int7 integrase

This part can be placed downstreamed a prmoter. It is sure that when it placed under a Ptac promoter on a P15A plasmid and full induced, the recombination between Int7 attP and attB will happen as expected. The RiboJ(BBa_K2615996) part was added before the RBS-coding sequence, so that the modularity and reuseability would be improved.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 389
    Illegal PstI site found at 938
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 80
    Illegal PstI site found at 389
    Illegal PstI site found at 938
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 268
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 389
    Illegal PstI site found at 938
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 389
    Illegal PstI site found at 938
    Illegal AgeI site found at 929
    Illegal AgeI site found at 1330
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/recombination
Parameters
None