Part:BBa_K2922034

The colicin-N operon under T7 promoter control

Summary

This is a composite part consist of a T7 promoter (BBa_K525998), CDS of colicin-N (BBa_K2922027), CDS of colicin-N immunity protein (BBa_K2922028) and CDS of lysis protein (BBa_K2922029). Each CDS has an RBS (BBa_B0034) behind. T7 promoter could be induced by IPTG or lactose in Escherichia coli BL21 strain and then express all proteins mentioned. E coli that can`t express colicin-N immunity protein would be killed by colicin-N, colicin-N immunity protein is used to protect itself from attack of extracellular colicin-N and lysis protein helps the release of colicin-N. This part is constructed in the aim of achieve our “aggressive” design.

The diagram of Colicin-N kit design

Ientification

To build this part correctly, Agarose gel electrophoresis is used to examine and strands in correct length are extracted to sequence for further examination.

Fig.1 The Agarose gel electrophoresis result for BBa_K2922034, plasmid that containing this part is digested by EcoRI and PstI.Strands in red box are fragments of interest.

In order to quantify its biological function, Plasmid pSB1C3 are used to carried this part and this plasmid are transformed into E coli BL21 (DE3) strain. Clonies were picked and cultured in liquid LB medium, then we used the supernatant and precipitate in our inhibition zone experiment. The detail of the protocol can be viewed in Notebook-Experiment-Inhibition zone. Results of the inhibition zone are shown below.

Fig.2 The inhibition zone test experiment of E coli BL21 (DE3) strain carrying BBa_K2922034. IPTG was added before for fully expression of colicin and immunity proteins. 100μL supernatant were added into oxford cup.

This result indicated that colicin could be expressed and released into supernatant, and it also showed the ability to kill other E coli strains without immunity proteins. The inhibition zone occurred in the control group means this promoter may be leaky.

For more information, please go to our result

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 598
  Illegal AgeI site found at 1602
  
• 1000
  COMPATIBLE WITH RFC[1000]