Composite

Part:BBa_K2922034

Designed by: Yunyue Shi   Group: iGEM19_XMU-China   (2019-10-13)

The colicin-N operon under T7 promoter control

Summary

This is a composite part consist of a T7 promoter (BBa_K525998), CDS of colicin-N (BBa_K2922027), CDS of colicin-N immunity protein (BBa_K2922028) and CDS of lysis protein (BBa_K2922029). Each CDS has an RBS (BBa_B0034) behind. T7 promoter could be induced by IPTG or lactose in Escherichia coli BL21 strain and then express all proteins mentioned. E coli that can`t express colicin-N immunity protein would be killed by colicin-N, colicin-N immunity protein is used to protect itself from attack of extracellular colicin-N and lysis protein helps the release of colicin-N. This part is constructed in the aim of achieve our “aggressive” design.


Ientification



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 598
    Illegal AgeI site found at 1602
  • 1000
    COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None