Composite

Part:BBa_K3110030

Designed by: Mrugank Dake   Group: iGEM19_IISER_Tirupati   (2019-10-14)
Revision as of 18:40, 14 October 2019 by Registry (Talk | contribs)

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Weak Promoter Weak RBS lldP+lldD

L-Lactate Dehydrogenase (lldD) and L-Lactate Permease (lldP) under the control of a weak promoter and a weak RBS.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 327
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2857
    Illegal AgeI site found at 463
    Illegal AgeI site found at 926
    Illegal AgeI site found at 1364
    Illegal AgeI site found at 1523
    Illegal AgeI site found at 1535
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2294


[edit]
Categories
Parameters
None