Composite

Part:BBa_K3110030

Designed by: Mrugank Dake   Group: iGEM19_IISER_Tirupati   (2019-10-14)


Weak Promoter Weak RBS lldP+lldD

This construct has L-Lactate Dehydrogenase (lldD) and L-Lactate Permease (lldP) under the control of a weak promoter and a weak RBS. lldD cleaves L-Lactate to pyruvate and lldP permits the entry of L-Lactate inside the bacterial cell. Our intention of using this part is to have control over the detection threshold of L-Lactate by the lldPRD regulatory region. We have also designed other parts which produce various higher and lower concentrations of lldD and lldP by varying the strength of the promoter and RBS controlling their production.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 327
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2857
    Illegal AgeI site found at 463
    Illegal AgeI site found at 926
    Illegal AgeI site found at 1364
    Illegal AgeI site found at 1523
    Illegal AgeI site found at 1535
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2294


Characterisation

The construct was not characterised due to insufficient time.

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Categories
Parameters
None