Part:BBa_K3102015

HLYA is a signal peptide found in the C-terminal signal sequence of alpha-hemolysin. It is used to target proteins for secretion via the Type I secretion pathway of gram-negative bacteria [Nicaud J. M. et al., 1986; Stanley P. et al., 1991]. Unlike Type II signal peptides, fusion of the HlyA signal peptide to the target protein result in transport of the protein from the cytoplasm to the extracellular medium in a single step.

For our project, this protein is used to secrete both CAE and EG1 proteins by E.coli.