Tag

Part:BBa_K3102015

Designed by: Sarah DUQUESNE   Group: iGEM19_Ionis_Paris   (2019-10-03)


hlyA (Hemolysin A)

HLYA is a signal peptide found in the C-terminal signal sequence of alpha-hemolysin. It is used to target proteins for secretion via the Type I secretion pathway of gram-negative bacteria [Nicaud J. M. et al., 1986; Stanley P. et al., 1991]. Unlike Type II signal peptides, fusion of the HlyA signal peptide to the target protein result in transport of the protein from the cytoplasm to the extracellular medium in a single step.

For our project, this protein is used to secrete both CAE and EG1 proteins by E.coli.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 112
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 112
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 112
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 112
  • 1000
    COMPATIBLE WITH RFC[1000]


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