Tag
Part:BBa_K3102015
Designed by: Sarah DUQUESNE Group: iGEM19_Ionis_Paris (2019-10-03)
hlyA (Hemolysin A)
HLYA is a signal peptide found in the C-terminal signal sequence of alpha-hemolysin. It is used to target proteins for secretion via the Type I secretion pathway of gram-negative bacteria [Nicaud J. M. et al., 1986; Stanley P. et al., 1991]. Unlike Type II signal peptides, fusion of the HlyA signal peptide to the target protein result in transport of the protein from the cytoplasm to the extracellular medium in a single step.
For our project, this protein is used to secrete both CAE and EG1 proteins by E.coli.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 112
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 112
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 112
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 112
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
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