Composite

Part:BBa_K3021004:Design

Designed by: Jeremy Hu   Group: iGEM19_PuiChing_Macau   (2019-10-14)
Revision as of 00:24, 15 October 2019 by Registry (Talk | contribs)

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NSP4-Laccase1326-HisTag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1493
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1007
    Illegal BamHI site found at 1526
    Illegal XhoI site found at 227
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 136
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 862


Design Notes

In these, we have further optimized the Laccase and NSP4 sequence.


Source

The original NSP4 sequence from the paper is ATGAAAAAGATTACCGCTGCTGCTGGTCTGCTGCTCCTCGCTGCCCAGCCGGCGATGGCG with the Protein sequence is The n-region: MKKI(the positive net charge in the n-region: +2) The h-region: TAAAGLLLLA The c-region: AQPAMA; We have codon optimized this sequence and also the Laccase1326 sequence, which is from previous publication.

References