Coding

Part:BBa_K2923009

Designed by: Kateryna LEN   Group: iGEM19_Strasbourg   (2019-10-13)
Revision as of 11:54, 13 October 2019 by Registry (Talk | contribs)

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LexA WT repressor

LexA (22.7 kDa) is a transcriptional repressor involved in SOS-system (a DNA damage response) by its binding with a promoter sequence of SOS-response regulatory genes. This LexA repressor sequence was previously described in Dimitrova et al. 1998 as part of the new LexA-based genetic system for monitoring and analyzing protein heterodimerization. The detection process involves two fusions proteins, which are two versions of chimeric LexA dimer. This sequence corresponds to wild type LexA repressor, recognizing LexA operator sequence CTGT.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 249
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds
Parameters
protein