Part:BBa_K2923009
LexA WT repressor
LexA (22.7 kDa) is a transcriptional repressor involved in SOS-system (a DNA damage response) by its binding with a promoter sequence of SOS-response regulatory genes. This LexA repressor sequence was previously described in Dimitrova et al. 1998 as part of the new LexA-based genetic system for monitoring and analyzing protein heterodimerization. The detection process involves two fusions proteins, which are two versions of chimeric LexA dimer. This sequence corresponds to wild type LexA repressor, recognizing LexA operator sequence CTGT.
Usage and Biology
This LexA WT corresponds to LexA coding sequence (85 amino acids) WT from E. coli strain JL1434 [Dimitrova, M., Younès-Cauet, G., Oertel-Buchheit, P., Porte, D., Schnarr, M., and Granger-Schnarr, M. (1998). A new LexA-based genetic system for monitoring and analyzing protein heterodimerization in Escherichia coli. Molecular and General Genetics MGG 257, 205–212.]
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 249
- 1000COMPATIBLE WITH RFC[1000]
protein |