RNA

Part:BBa_K2923003

Designed by: claire husser   Group: iGEM19_Strasbourg   (2019-09-12)
Revision as of 11:17, 12 September 2019 by Registry (Talk | contribs)

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Guanine actif aptazyme

an aptazyme is an aptamer-ribozyme combinations with intrinsic RNA cleavage activity. this aptazyme is specific for the guanine (liguant). He is composed in 3 differents part, a twister ribozyme module, a communication module and an aptamer module. This aptazyme is a ON-switches aptazyme, this mean that in the absence of liguant the RNA does not self-cleave and in the presence of liguant the RNA self-cleaves. This mean that you can sense the guanine in a medium with this aptazyme.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//classic/rna/uncategorized
Parameters
ligandsin-vitro assay : Guanine; in-vivo assay Guanosine
switch_pointThe maximum effect obtained at approximately 500 μM of guanosine