Plasmid backbones/Version 5/Features
Revision as of 01:46, 7 September 2008 by Rshetty (Talk | contribs) (Plasmids/Reshma plasmid features moved to Plasmids/Version 5 plasmid/Features)
- A complete BioBrick® cloning site for easy cloning and assembly of BioBrick® parts.
- A ccdB positive selection marker within the BioBrick® cloning site. The ccdB gene ensures that when assembling two BioBrick® parts together, the uncut plasmid vector is not transformed. However, inclusion of the ccdB gene means that these vectors must be propagated in a ccdB tolerant strain, such as E. coli strain DB3.1 (BBa_V1005).
- A low or medium copy replication origin to reduce consumption of cellular resources during device or system operation.
- Forward and reverse terminators both upstream and downstream of the BioBrick® cloning site to insulate the vector from read-through transcription originating in the cloned BioBrick® device or system and to insulate the cloned BioBrick® system from the vector.
- Stop codons in all reading frames to prevent inadvertent translation into or out of the BioBrick cloning site.
- Primer binding sites for the standard BioBrick® verification primers VF2 (BBa_G00100) and VR (BBa_G00101). These primers are located for convenient sequencing and [http://openwetware.org/wiki/Colony_PCR screening by colony PCR] of cloned BioBrick® devices and systems.