Composite

Part:BBa_K3257100

Designed by: Yi Qiao   Group: iGEM19_Fudan-TSI   (2019-09-23)
Revision as of 17:43, 23 September 2019 by YY12138 (Talk | contribs)

Improved LacI Expression System

This part is the system we build to express our improved LacI. With lacIq promoter and rrnB T1 terminator, improved LacI protein can be expressed and function properly in the Escherichia coli BL21(DE3). We used EGFP as a reporter controlled by our improved Lac operon and measured its green fluorescence over time.

According to our experiment, our Lac operon is improved in the following three main aspects.

Lower Response to Arabinose, Better Orthogonality

Same Level of Expression when Induced by IPTG

Lower Uninduced Leakage


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1258
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1258
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1258
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

AJ Meyer et al. Escherichia coli "Marionette" strains with 12 highly optimized small-molecule sensors. Nat Chem Biol. 2019 Feb;15(2):196-204. doi: 10.1038/s41589-018-0168-3. Epub 2018 Nov 26.


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