Coding

Part:BBa_K2660009

Designed by: Danielle Biscaro Pedrolli, Victor Nunes de Jesus   Group: iGEM18_Unesp_Brazil   (2018-10-08)
Revision as of 13:06, 14 October 2018 by VictorNJ (Talk | contribs)


pMag - Light-activated protein

This photoswitch made from a blue light photoreceptor VVD derived from the filamentous fungus Neurospora crassa. The VVD is currently one of the smallest photoreceptors among those tested as optogenetic tools.The positive Magnet (pMag) when irratiate by blue ligth



This part can be used with nMag (BBa_K2660008) to join different domains of a protein of interest when irradiated by blue light


References
Kawano, F. et al. Engineered pairs of distinct photoswitches for optogenetic control of cellular proteins. Nat. Commun. 6:6256 doi: 10.1038/ncomms7256 (2015)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 87
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/bsubtilis
//collections/probiotics/biocontainment
Parameters
None