1 Registry Star
Generator
BSMT1
Part:BBa_J45119:Experience
Designed by: Boyuan Zhu Group: iGEM06_MIT (2006-08-10)
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Applications of BBa_J45119
Wintergreen odor based reporter of transcriptional signal.
User Reviews
UNIQc9e479edb7a089c3-partinfo-00000000-QINU
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BBa_J45119 was successfully used to produce wintergreen odor under the control of BBa_R0040 in the wintergreen odor generator BBa_J45120. |
UNIQc9e479edb7a089c3-partinfo-00000005-QINU
Characterization
Transcriptional control of wintergreen odor generator
The BSMT1 enzyme generator is used in both the BBa_J45120 and BBa_J45181 wintergreen odor biosynthetic devices.
Growth phase dependent wintergreen odor production
To demonstrate growth phase dependent wintergreen odor production, we compared the behavior of constitutive and exponential phase dependent wintergreen odor generators (A and B, respectively). We measured methyl salicylate concentration (relative to the pentachloronitrobenzene internal standard) of cultures of the constitutive and exponential phase wintergreen odor generators at different culture cell densities (OD600nm) (C). The constitutive and exponential phase wintergreen odor generators produced similar levels of methyl salicylate at all cell densities examined. Thus, the exponential phase wintergreen odor generator does not work as intended.
To demonstrate growth phase dependent wintergreen odor production, we compared the behavior of constitutive and exponential phase dependent wintergreen odor generators (A and B, respectively). We measured methyl salicylate concentration (relative to the pentachloronitrobenzene internal standard) of cultures of the constitutive and exponential phase wintergreen odor generators at different culture cell densities (OD600nm) (C). The constitutive and exponential phase wintergreen odor generators produced similar levels of methyl salicylate at all cell densities examined. Thus, the exponential phase wintergreen odor generator does not work as intended.